Device

Part:BBa_K5250025:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter, RNAP + pT7-like, RFP + pT7-like, GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2715
    Illegal BamHI site found at 3510
    Illegal BamHI site found at 4287
    Illegal BamHI site found at 5167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2898
    Illegal AgeI site found at 3359
    Illegal AgeI site found at 4148
    Illegal AgeI site found at 4260
    Illegal AgeI site found at 5028
    Illegal AgeI site found at 5140
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1220
    Illegal BsaI site found at 2090
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 600
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 2538
    Illegal SapI site found at 2519
    Illegal SapI.rc site found at 1714


Design Notes

The basic part sequences were codon optimized.


Source

The sequences for the T7-like RNAP and promoter are taken from a Pseudomonas phage Pf-10. The rest of the basic part sequences are taken from the iGEM Parts Registry.

References